CHEK1 anticorps (pSer296) (HRP)

N° du produit ABIN1407914

L’anticorps Lapin Polyclonal anti-CHEK1 a été validé pour WB, ELISA, IHC (p) et IHC (fro). Il convient pour détecter CHEK1 dans des échantillons de Humain.

Aperçu rapide pour CHEK1 anticorps (pSer296) (HRP) (ABIN1407914)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CHEK1 anticorps (HRP)

Épitope: pSer296

Réactivité croisée: Humain

Homologie: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human CHEK1 isoform b around the phosphorylation site of Ser296

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHEK1

Sujet:

Synonyms: Chk1 Ser296, Chk1 phospho S296,Chk1 Ser296, p-Chk1 phospho S296, Chk1S296, Cell cycle checkpoint kinase, Checkpoint, S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog S. pombe, CHEK 1, CHEK-1, CHEK1, Chk 1, Chk1, CHK1 checkpoint homolog S. pombe, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A S. cerevisiae, C85740, CHK1_HUMAN.

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011].

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN